Preferential activation of [3H]phorbol-12,13-dibutyrate binding by AMPA (alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid) in neonatal striatal cell cultures.

Article date: 1992/10/16

PubMed ID: 1280525

Journal name: Brain research (ISSN: 0006-8993)


Activation of excitatory amino acid receptors increased [3H]phorbol-12,13-dibutyrate ([3H]PdBu) binding in four week cultures of striatal cells from postnatal day 7 rat pups (PN7), and in PN7 cells co-cultured the fourth week with striatal cells from postnatal day 1 rat pups. Kainate (KA), trans-1-amino-cyclopentyl-1,3-dicarboxylate (ACPD), and N-methyl-D-aspartate (NMDA) increased [3H]PdBu binding equally in both types of cultures, but alpha-amino-3-hydroxy-5-methyl-4-isoxazole-propionic acid (AMPA) increased binding by 3-fold in the co-cultures. Thus, [3H]PdBu binding in these two types of striatal cultures offers a simple model system for studying the regulation of AMPA/KA receptor responses.

Author List: McMillian M, Hong J S, Pennypacker K R

Publication Types: Journal Article

Substances mentioned in the article: Glial Fibrillary Acidic Protein; Neurotoxins; Cycloleucine; 1-amino-1,3-dicarboxycyclopentane; Ibotenic Acid; Phorbol 12,13-Dibutyrate; N-Methylaspartate; alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid; Phosphopyruvate Hydratase; Kainic Acid;

Mesh terms: Animals; Animals, Newborn; Binding Sites; Cells, Cultured; Corpus Striatum/metabolism; Cycloleucine/analogs & derivatives; Glial Fibrillary Acidic Protein/analysis; Ibotenic Acid/analogs & derivatives; Kainic Acid/pharmacology; N-Methylaspartate/pharmacology; Neurons/cytology; Neurotoxins/pharmacology; Phorbol 12,13-Dibutyrate/metabolism; Phosphopyruvate Hydratase/analysis; Rats; Rats, Inbred F344; alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid;

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