Article date: 1992/9/1
PubMed ID: 1280546
Journal name: Comparative biochemistry and physiology. C, Comparative pharmacology and toxicology (ISSN: 0742-8413)
1. The receptor for the neurotransmitter glutamate was functionally expressed in oocytes of Xenopus laevis after microinjection of rat brain RNA. The functional differentiation of this receptor type was further analyzed. 2. The development of the sensitivity to the agonists showed a time course which was differential for the various ligands in individual oocytes. 3. Sensitivity appeared after one day for kainate (KA), after two days for alpha-amino-3-hydroxy-5-methyl-4-isoxazole-propionate (AMPA) and quisqualate (QA), and after five days for N-methyl-D-aspartate (NMDA). 4. The KA response was markedly reduced by simultaneous application of AMPA. This was even found on the first day when an agonistic AMPA reaction was not detectable. 5. NMDA and non-NMDA receptors can clearly be differentiated by their delay of expression.
Author List: Musshoff U, Madeja M, Bloms P, Speckmann E J
Publication Types: Journal Article
Substances mentioned in the article: RNA, Messenger; Receptors, Glutamate; Ibotenic Acid; N-Methylaspartate; alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid; Quisqualic Acid; Kainic Acid;
Mesh terms: Animals; Cerebral Cortex/chemistry; Electrophysiology; Ibotenic Acid/analogs & derivatives; Kainic Acid/pharmacology; N-Methylaspartate/pharmacology; Oocytes/metabolism; Quisqualic Acid/pharmacology; RNA, Messenger/genetics; Rats; Receptors, Glutamate/biosynthesis; Xenopus laevis/metabolism; alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid;