1282226

Evans blue reduces macroscopic desensitization of non-NMDA receptor mediated currents and prolongs excitatory postsynaptic currents in cultured rat thalamic neurons.

Article date: 1992/10/26

PubMed ID: 1282226

Journal name: Neuroscience letters (ISSN: 0304-3940)

ABSTRACT

Fast application of L-glutamate, AMPA (alpha-amino-3-hydroxy-5-methylisoxazole-4-propionic acid) or kainate to cultured rat thalamic neurons revealed properties of non-NMDA (N-methyl-D-aspartate) receptors similar to those described in hippocampal neurons. The kinetics of non-NMDA receptor-mediated currents were altered by the addition of the dye Evans Blue (EB). Macroscopic desensitization was reduced and activation and deactivation kinetics were slowed. Delayed addition of EB, after desensitization of non-NMDA receptors, resulted in reactivation of desensitized receptors. Thus, both ion channel gating and entry into the desensitized state were affected. Evans blue also slowed the activation and the decay of glutamatergic miniature EPSCs (excitatory postsynaptic currents), demonstrating that receptor kinetics determine the time course of the synaptic response.

Author List: Lessmann V, Gottmann K, Lux H D

Publication Types: Journal Article; Research Support, Non-U.S. Gov't

Substances mentioned in the article: Glutamates; Receptors, Amino Acid; Ibotenic Acid; Evans Blue; alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid; Quisqualic Acid;

Mesh terms: Animals; Evans Blue/pharmacology; Female; Glutamates/metabolism; Ibotenic Acid/analogs & derivatives; Ion Channel Gating/drug effects; Neurons/metabolism; Pregnancy; Quisqualic Acid/pharmacology; Rats; Receptors, Amino Acid/drug effects; Synapses/drug effects; Thalamus/cytology; alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid;

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