Article date: 1992/1/1
PubMed ID: 1309240
Journal name: Journal of neurochemistry (ISSN: 0022-3042)
Antibodies raised against the synthetic peptide NH2-QKSDDDYEDYASNKTC-COOH (gamma 2 1-15 Cys), which corresponds to the N-terminal amino acid sequence with a C-terminal cysteine of the human gamma 2 subunit of the gamma-aminobutyric acidA (GABAA) receptor, were used to study the quantitative immunoprecipitation of agonist benzodiazepine binding sites from bovine brain. Anti-gamma 2 1-15 Cys antibodies were found to immunoprecipitate specifically in parallel [3H]flunitrazepam- and [3H]muscimol-reversible binding sites in a dose-dependent manner. The maximum percentages of [3H]flunitrazepam binding sites immunoprecipitated from detergent extracts of bovine cerebral cortex, cerebellum, and hippocampus were 68, 77, and 83%, respectively. Immunoprecipitation studies with anti-alpha 1 324-341 antibodies carried out in parallel with anti-gamma 2 1-15 Cys antibodies provided evidence for the promiscuity of the gamma 2 subunit within native GABAA receptors. These results substantiate the association of the gamma 2 polypeptide with native GABAA receptors.
Author List: Duggan M J, Pollard S, Stephenson F A
Publication Types: Journal Article; Research Support, Non-U.S. Gov't
Substances mentioned in the article: Antibodies; Peptide Fragments; Receptors, GABA-A; Muscimol; Flunitrazepam;
Mesh terms: Amino Acid Sequence; Animals; Antibodies/immunology; Binding Sites; Brain/metabolism; Cattle; Flunitrazepam/metabolism; Molecular Sequence Data; Muscimol/metabolism; Peptide Fragments/immunology; Precipitin Tests/methods; Receptors, GABA-A/chemistry; Tissue Distribution;