Muscimol-induced reduction of GABAA receptor alpha 1-subunit mRNA in primary cultured cerebral cortical neurons.

Article date: 1992/10/1

PubMed ID: 1331688

Journal name: Brain research. Molecular brain research (ISSN: 0169-328X)


The expression of mRNA for GABAA receptor alpha 1-subunit in mouse cerebral cortical neurons in primary culture was examined using RNA blot analysis and ribonuclease protection assay following the treatment of neurons with muscimol, a selective agonist of GABAA receptor. The level of mRNA for GABAA receptor alpha 1-subunit showed a decrease in comparison with that in non-treated cells, whereas no changes in the level of beta-actin mRNA were noted under the same experimental conditions. This muscimol-induced reduction in GABAA receptor alpha 1-subunit mRNA was counteracted by the simultaneous exposure of neurons to both bicuculline, an antagonist of GABAA receptor, and muscimol. The expression of mRNA for GABAA receptor alpha 1-subunit also showed a decline by the treatment of cells with flunitrazepam alone, an agonist of benzodiazepine receptor, and this change was also abolished by the simultaneous exposure of cells to flunitrazepam and Ro15-1788, an antagonist for central benzodiazepine receptor. These results suggest that the continuous stimulation of cerebral GABAA receptor complex may induce the reduced expression of mRNA for the receptor complex.

Author List: Hirouchi M, Ohkuma S, Kuriyama K

Publication Types: Journal Article; Research Support, Non-U.S. Gov't

Substances mentioned in the article: Peptide Fragments; RNA, Messenger; Receptors, GABA-A; Muscimol; Flunitrazepam; Ribonucleases; Bicuculline;

Mesh terms: Animals; Bicuculline/pharmacology; Blotting, Northern; Cells, Cultured; Cerebral Cortex/drug effects; Flunitrazepam/pharmacology; Mice; Muscimol/pharmacology; Neurons/drug effects; Peptide Fragments/genetics; RNA, Messenger/drug effects; Receptors, GABA-A/drug effects; Ribonucleases;

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