Article date: 1992/2/14
PubMed ID: 1351782
Journal name: Brain research (ISSN: 0006-8993)
The effect of a series of glutamate uptake inhibitors was tested on ibotenate-stimulated phosphoinositide hydrolysis. The pharmacological profile of the inhibitory effect of these compounds on the ibotenate response was quite different from that on glutamate uptake. Aspartate-beta-hydroxamate was the most potent compound with the L-isomer (IC50 11 +/- 2 microM) being considerably more potent than the D-isomer (IC50 104 +/- 12 microM). The effect of the L-aspartate-beta-hydroxamate was found to be specific for ibotenate and quisqualate-stimulated phosphoinositide hydrolysis; this compound did not affect hydrolysis stimulated by carbachol, K+ or sodium fluoride. The inhibition of the ibotenate response was found to involve a non-competitive and irreversible mechanism.
Author List: Ormandy G C
Publication Types: Journal Article
Substances mentioned in the article: Glutamates; Phosphatidylinositols; beta-aspartylhydroxamic acid; Ibotenic Acid; Glutamic Acid; Asparagine; Carbachol;
Mesh terms: Animals; Asparagine/analogs & derivatives; Carbachol; Glutamates/metabolism; Glutamic Acid; Hippocampus/drug effects; Hydrolysis; Ibotenic Acid/antagonists & inhibitors; In Vitro Techniques; Male; Phosphatidylinositols/metabolism; Rats; Rats, Inbred Strains;