1351782

Inhibition of excitatory amino acid-stimulated phosphoinositide hydrolysis in rat hippocampus by L-aspartate-beta-hydroxamate.

Article date: 1992/2/14

PubMed ID: 1351782

Journal name: Brain research (ISSN: 0006-8993)

ABSTRACT

The effect of a series of glutamate uptake inhibitors was tested on ibotenate-stimulated phosphoinositide hydrolysis. The pharmacological profile of the inhibitory effect of these compounds on the ibotenate response was quite different from that on glutamate uptake. Aspartate-beta-hydroxamate was the most potent compound with the L-isomer (IC50 11 +/- 2 microM) being considerably more potent than the D-isomer (IC50 104 +/- 12 microM). The effect of the L-aspartate-beta-hydroxamate was found to be specific for ibotenate and quisqualate-stimulated phosphoinositide hydrolysis; this compound did not affect hydrolysis stimulated by carbachol, K+ or sodium fluoride. The inhibition of the ibotenate response was found to involve a non-competitive and irreversible mechanism.

Author List: Ormandy G C

Publication Types: Journal Article

Substances mentioned in the article: Glutamates; Phosphatidylinositols; beta-aspartylhydroxamic acid; Ibotenic Acid; Glutamic Acid; Asparagine; Carbachol;

Mesh terms: Animals; Asparagine/analogs & derivatives; Carbachol; Glutamates/metabolism; Glutamic Acid; Hippocampus/drug effects; Hydrolysis; Ibotenic Acid/antagonists & inhibitors; In Vitro Techniques; Male; Phosphatidylinositols/metabolism; Rats; Rats, Inbred Strains;

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