Article date: 1990/3/1
PubMed ID: 1369647
Journal name: Medical journal of Osaka University (ISSN: 0030-6169)
We previously reported that zonisamide inhibits both [3H]flunitrazepam and [3H]muscimol binding in rat brain. In the present study, [3H]zonisamide was found to bind in a saturable fashion to the crude synaptosomal fraction of whole rat brain. Linear regression analysis of the binding data in the Scatchard plot indicated a Kd of 90 nM, and a maximal binding capacity of 1.40 x 10(3) fmol/mg protein. Displacement studies revealed an inhibitory effect of clonazepam and an enhancement effect of GABA on specific [3H]zonisamide binding. These results suggest that specific [3H]zonisamide binding sites may have a tight correlationship with benzodiazepine receptors in rat brain.
Author List: Mimaki T, Suzuki Y, Tagawa T, Karasawa T, Yabuuchi H
Publication Types: Journal Article; Research Support, Non-U.S. Gov't
Substances mentioned in the article: Anticonvulsants; Isoxazoles; Receptors, GABA-A; zonisamide; gamma-Aminobutyric Acid; Clonazepam;
Mesh terms: Animals; Anticonvulsants/metabolism; Brain/metabolism; Clonazepam/pharmacology; Dose-Response Relationship, Drug; Isoxazoles/metabolism; Male; Rats; Rats, Sprague-Dawley; Receptors, GABA-A/metabolism; Synaptosomes/metabolism; gamma-Aminobutyric Acid/pharmacology;