1373796

Calcium directly permeates kainate/alpha-amino-3-hydroxy-5-methyl-4- isoxazolepropionic acid receptors in cultured cerebellar Purkinje neurons.

Article date: 1992/4/1

PubMed ID: 1373796

Journal name: Molecular pharmacology (ISSN: 0026-895X)

ABSTRACT

In cultures of rat cerebellar neurons that were enriched in Purkinje cells, the non-N-methyl-D-aspartate glutamate receptor agonist kainate (KA) stimulated Ca2+ influx into all neurons in Na(+)-containing solutions. A large Ca2+ influx was also observed in most neurons when KA was applied in Na(+)-free solutions, even when the cells were voltage-clamped at negative potentials. KA also stimulated Co2+ uptake into both Purkinje and non-Purkinje neurons. The KA-induced Ca2+ influx was insensitive to pharmacological antagonists of voltage-sensitive Ca2+ channels and antagonists of N-methyl-D-aspartate receptors. Thus, different types of cerebellar neurons possess KA-gated ionophores that are permeable to Ca2+. This Ca2+ conductance may play an important role in glutamate-mediated physiological and pathological events in the cerebellum.

Author List: Brorson J R, Bleakman D, Chard P S, Miller R J

Publication Types: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.

Substances mentioned in the article: Receptors, AMPA; Receptors, Kainic Acid; Receptors, N-Methyl-D-Aspartate; Receptors, Neurotransmitter; Ibotenic Acid; alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid; Kainic Acid; Calcium;

Mesh terms: Animals; Biological Transport; Calcium/metabolism; Cells, Cultured; Fluorescent Antibody Technique; Ibotenic Acid/analogs & derivatives; Kainic Acid/metabolism; Membrane Potentials; Purkinje Cells/metabolism; Rats; Receptors, AMPA; Receptors, Kainic Acid; Receptors, N-Methyl-D-Aspartate/drug effects; Receptors, Neurotransmitter/metabolism; alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid;

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