Choline acetyltransferase- and substance P-like immunoreactive elements in fetal striatal grafts in the rat: a correlated light and electron microscopic study.

Article date: 1992/1/11

PubMed ID: 1374856

Journal name: Neuroscience (ISSN: 0306-4522)


Fetal striatal neurons were transplanted into the ibotenic acid-lesioned rat striatum. Three months after transplantation, the graft tissue was processed for choline acetyltransferase- and substance P-like immunoreactivity and was subsequently examined at the light and electron microscopic levels. The study demonstrated that choline acetyltransferase- and substance P-like-immunoreactive neurons were homogenously present throughout fetal striatal grafts, although in decreased numbers compared with those in the normal rat striatum. The majority of the choline acetyltransferase-immunoreactive neurons had fusiform, oval, or polygonal somata with somatic diameters greater than 20 microns and contained deeply invaginated nuclei surrounded by copious cytoplasm. In addition, choline acetyltransferase-immunoreactive neurons with somatic diameters between 10 and 20 microns were also demonstrated. The grafts' substance P-like-immunoreactive neurons, which had somatic diameters between 10 and 25 microns and had oval or polygonal perikarya, could be classified into two types based on their ultrastructural characteristics. Type I neurons contained an unindented nucleus which was surrounded by a thin rim or moderate amount of cytoplasm, whereas Type II immunoreactive neurons contained an indented nucleus which was surrounded by copious cytoplasm. Choline acetyltransferase- and substance P-like-immunoreactive dendrites in the grafts' neuropil were contacted by multiple unlabeled axon terminals. In addition, choline acetyltransferase- and substance P-like-immunoreactive axon terminals forming symmetric contacts with unlabeled dendrites were present within the graft. The study demonstrated that many of the neuroanatomical features of choline acetyltransferase- and substance P-like-immunoreactive elements found in the normal rat striatum are present in mature fetal striatal grafts.

Author List: Helm G A, Palmer P E, Bennett J P

Publication Types: Journal Article

Substances mentioned in the article: Substance P; Choline O-Acetyltransferase;

Mesh terms: Animals; Axons/enzymology; Brain Tissue Transplantation/physiology; Choline O-Acetyltransferase/metabolism; Corpus Striatum/immunology; Dendrites/enzymology; Female; Fetal Tissue Transplantation/physiology; Immunohistochemistry; Microscopy, Electron; Nerve Endings/enzymology; Rats; Rats, Inbred Strains; Substance P/immunology;

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