The effects of kainate and the glutamate agonist, AMPA, are not separable in rat neocortical cultures.

Article date: 1992/7/6

PubMed ID: 1380682

Journal name: Neuroscience letters (ISSN: 0304-3940)


The excitotoxic and [3H]gamma-aminobutyric acid ([3H]GABA)-releasing effects of quisqualate, alpha-amino-3-hydroxy-4-methyl-5-isoxazolepropionic acid (AMPA), kainate (KA), and their combinations were examined in primary cultures of the rat cerebral cortex. [3H]GABA efflux was evoked by a 5 min exposure of preloaded cultures to the respective agonist(s) (0.5 mM each). Cell death was induced by a 24 h exposure of cells to 1 mM quisqualate, AMPA and/or KA, and was quantified by measuring lactic dehydrogenase leakage. When applied alone, each agonist induced remarkable [3H]GABA release and excitotoxic cell death. Simultaneous administration of AMPA or quisqualate and KA did not evoke stronger responses than KA alone. These results indicate that AMPA and KA receptors are located on the same cortical cells and may activate the same receptor channels and/or intracellular messengers.

Author List: Cai N S, Erdö S L

Publication Types: Journal Article; Research Support, Non-U.S. Gov't

Substances mentioned in the article: Receptors, AMPA; Receptors, Kainic Acid; Receptors, Neurotransmitter; Ibotenic Acid; gamma-Aminobutyric Acid; alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid; Quisqualic Acid; L-Lactate Dehydrogenase; Kainic Acid;

Mesh terms: Animals; Cells, Cultured; Cerebral Cortex/cytology; Female; Ibotenic Acid/analogs & derivatives; Kainic Acid/pharmacology; L-Lactate Dehydrogenase/metabolism; Neurons/drug effects; Pregnancy; Quisqualic Acid/metabolism; Rats; Rats, Inbred Strains; Receptors, AMPA; Receptors, Kainic Acid; Receptors, Neurotransmitter/drug effects; alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid; gamma-Aminobutyric Acid/metabolism;

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