Presence of the binding of a variety of ligands related to ionotropic excitatory amino acid receptors in rat retina.

Article date: 1992/3/27

PubMed ID: 1381259

Journal name: Brain research (ISSN: 0006-8993)


The binding of [3H]L-glutamic (Glu), [3H](+/-)-3-(2-carboxypiperazin-4-yl)propyl-1-phosphonic and [3H]D,L-(E)-2-amino-4-propyl-5-phosphono-3-pentenoic acids was detected in rat retinal membranes extensively washed and treated with a low concentration of Triton X-100, in addition to the binding of both [3H]glycine (Gly) and [3H]5,7-dichlorokynurenic acid. Furthermore, retinal membranes exhibited the binding of [3H](+)-5-methyl-10,11-dihydro-5H-dibenzo[a,d]cyclohepten-5,10-imi ne in the presence of Glu, Gly and spermidine irrespective of the incubation period employed. Rat retina also contained the binding of [3H]kainic acid as well as the binding of [3H]alpha-amino-3-hydroxy-5-methylisoxazole-4-propionic acid which was sensitive to potentiation by potassium thiocyanate. In addition, the binding of both [3H](+)-3-(hydroxyphenyl)-N-(l-propyl)piperidine and [3H]1,3-di-o-tolyl-guanidine was found in rat retinal membranes extensively washed but not treated with Triton X-100. These results give support for the proposal that the rodent retina contains subclasses of ionotropic brain excitatory amino acid receptors including the N-methyl-D-aspartate receptor ionophore complex as well as sigma sites.

Author List: Zuo P, Ogita K, Yoneda Y

Publication Types: Journal Article; Research Support, Non-U.S. Gov't

Substances mentioned in the article: Receptors, AMPA; Receptors, Glycine; Receptors, N-Methyl-D-Aspartate; Receptors, Neurotransmitter; Tritium; Ibotenic Acid; alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid; Glycine;

Mesh terms: Animals; Cell Membrane/metabolism; Glycine/metabolism; Ibotenic Acid/analogs & derivatives; Kinetics; Male; Radioligand Assay; Rats; Rats, Inbred Strains; Receptors, AMPA; Receptors, Glycine; Receptors, N-Methyl-D-Aspartate/metabolism; Receptors, Neurotransmitter/metabolism; Retina/metabolism; Tritium; alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid;

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