1382660

Phospholipase A2-induced changes in AMPA receptor: an autoradiographic study.

Article date: 1992/6/1

PubMed ID: 1382660

Journal name: Neuroreport (ISSN: 0959-4965)

ABSTRACT

The expression of long-term potentiation and learning of a classical conditioning task increase [3H]-AMPA binding in hippocampus. Phospholipase A2 (PLA2) has been proposed to underly these changes, as PLA2 treatment of membrane preparations increases the affinity of AMPA receptors for agonists. We demonstrate here that preincubation of thin (10 microns) frozen rat brain sections with exogenous PLA2 and calcium at physiological temperature changes the binding properties of AMPA receptors. Quantitative autoradiography reveals that PLA2-treatment produces a differential increase in [3H]-AMPA binding across brain regions. The same treatment also decreases the binding of an antagonist ([3H]-CNQX) throughout the brain. We propose that PLA2 treatment results in a modification of the AMPA receptors which is regionally specific, probably due to different AMPA receptor subunit compositions.

Author List: Tocco G, Massicotte G, Standley S, Thompson R F, Baudry M

Publication Types: Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.

Substances mentioned in the article: Oxadiazoles; Quinoxalines; Receptors, AMPA; Receptors, Neurotransmitter; Tritium; Ibotenic Acid; 6-Cyano-7-nitroquinoxaline-2,3-dione; alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid; Phospholipases A; Phospholipases A2;

Mesh terms: 6-Cyano-7-nitroquinoxaline-2,3-dione; Animals; Autoradiography; Brain/drug effects; Ibotenic Acid/analogs & derivatives; Male; Oxadiazoles/metabolism; Phospholipases A/pharmacology; Phospholipases A2; Quinoxalines/metabolism; Rats; Rats, Wistar; Receptors, AMPA; Receptors, Neurotransmitter/drug effects; Tritium; alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid;

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