NMDA-induced release of nitric oxide potentiates aspartate overflow from cerebellar slices.

Article date: 1992/4/13

PubMed ID: 1383875

Journal name: Neuroscience letters (ISSN: 0304-3940)


We report that stimulation of neonatal rat cerebellar slices with N-methyl-D-aspartate (NMDA) release nitric oxide (NO) and also increased the release of aspartate. Inhibition of NMDA receptors with the specific antagonist, 3-((RS)-2-carboxypiperazin-4-yl)-propyl-1-phosphonic acid (CPP) prevented the NMDA-induced release of both NO and aspartate. Similar results were obtained with the inhibitor of NO synthase, NG-nitroargine (NG-ARG). The NO scavenger, haemoglobin prevented the release of aspartate. Under calcium-free conditions NMDA-induced aspartate release was abolished and NO release significantly reduced. These results indicate that NO has a physiological role in the release of aspartate.

Author List: Dickie B G, Lewis M J, Davies J A

Publication Types: Journal Article; Research Support, Non-U.S. Gov't

Substances mentioned in the article: Ibotenic Acid; omega-N-Methylarginine; Aspartic Acid; Nitric Oxide; N-Methylaspartate; alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid; Quisqualic Acid; Arginine; Magnesium; Kainic Acid;

Mesh terms: Animals; Arginine/analogs & derivatives; Aspartic Acid/metabolism; Cerebellum/drug effects; Ibotenic Acid/analogs & derivatives; In Vitro Techniques; Kainic Acid/pharmacology; Magnesium/pharmacology; N-Methylaspartate/pharmacology; Nitric Oxide/metabolism; Quisqualic Acid/pharmacology; Rats; Rats, Wistar; alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid; omega-N-Methylarginine;

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