Article date: 1992/6/8
PubMed ID: 1383893
Journal name: Neuroscience letters (ISSN: 0304-3940)
The regional distribution of radioactive ligand binding for different receptors of the gamma-aminobutyric acid A (GABAA)-benzodiazepine-picrotoxin chloride channel complex was measured on tissue section by autoradiography in brains taken from a genetic strain of Wistar rats with spontaneous absence-like seizures, the genetic absence epilepsy rats from Strasbourg (GAERS), and a control colony. The ligands employed included [3H]muscimol for high affinity GABA agonists sites; [3H]SR 95531 for the low-affinity GABA sites; [3H]flunitrazepam for the benzodiazepine sites; and [35S]t-butyl bicyclophosphorothionate (TBPS) for the picrotoxin site. There was no significant change between GAERS and control animals in [3H]flunitrazepam and [35S]TBPS binding. However, there was significantly decreased [3H]muscimol and [3H]SR 95531 binding in the CA2 region of the hippocampus of the GAERS. This was due to a decrease in Bmax of both [3H]muscimol and [3H]SR 95531 binding in the epileptic strain.
Author List: Snead O C, Depaulis A, Banerjee P K, Hechler V, Vergnes M
Publication Types: Journal Article; Research Support, U.S. Gov't, P.H.S.
Substances mentioned in the article: Bridged Bicyclo Compounds; Bridged Bicyclo Compounds, Heterocyclic; Chloride Channels; Chlorides; Convulsants; GABA-A Receptor Antagonists; GABA-benzodiazepine receptor-chloride ionophore complex; Ion Channels; Ionophores; Membrane Proteins; Pyridazines; Receptors, GABA-A; Sulfur Radioisotopes; Tritium; Muscimol; Flunitrazepam; tert-butylbicyclophosphorothionate; gabazine;
Mesh terms: Animals; Autoradiography; Brain/metabolism; Bridged Bicyclo Compounds/metabolism; Bridged Bicyclo Compounds, Heterocyclic; Chloride Channels; Chlorides/metabolism; Convulsants/metabolism; Epilepsy, Absence/genetics; Flunitrazepam/metabolism; GABA-A Receptor Antagonists; Ion Channels/metabolism; Ionophores/metabolism; Male; Membrane Proteins/metabolism; Muscimol/metabolism; Organ Specificity; Pyramidal Tracts/metabolism; Pyridazines/metabolism; Rats; Rats, Wistar; Receptors, GABA-A/metabolism; Sulfur Radioisotopes; Tritium;