The effects of ibotenate lesions of the median preoptic nucleus on experimentally-induced and circadian drinking behavior in rats.

Article date: 1992/5/15

PubMed ID: 1504809

Journal name: Brain research (ISSN: 0006-8993)


Male Sprague-Dawley rats were injected with either ibotenic acid or vehicle in the region of the median preoptic nucleus (MnPO) during methoxyflurane anesthesia. Later, the rats were tested for drinking responses elicited by angiotensin II (1 and 2 mg/kg s.c.) and hypertonic saline (3 and 6% w/v s.c.). Tests were conducted at 3 different phases of a 12:12 light-dark (LD) cycle (in the middle of the light phase and in the early and midportion of the dark phase). The rats with ibotenate lesions of the MnPO drank significantly less than vehicle-injected and lesion control groups regardless of when the tests were conducted. Subsequent monitoring of the diurnal rhythm of drinking, employing electrical lickometers, detected no difference between the rhythms of 4 rats with ibotenate lesions of the MnPO who failed to drink to homeostatic challenges and 4 vehicle-injected control rats. The results indicate that ibotenic acid lesions of the MnPO block drinking behavior stimulated by angiotensin II and hypertonic saline without disrupting the entrainment or pattern of ad libitum drinking.

Author List: Cunningham J T, Beltz T, Johnson R F, Johnson A K

Publication Types: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.

Substances mentioned in the article: Saline Solution, Hypertonic; Angiotensin II; Ibotenic Acid;

Mesh terms: Angiotensin II/pharmacology; Animals; Circadian Rhythm/drug effects; Drinking Behavior/drug effects; Ibotenic Acid; Injections, Subcutaneous; Male; Preoptic Area/drug effects; Rats; Rats, Inbred Strains; Saline Solution, Hypertonic;

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