1649249

Modulation of protein kinase C translocation by excitatory and inhibitory amino acids in primary cultures of neurons.

Article date: 1991/8/1

PubMed ID: 1649249

Journal name: Journal of neurochemistry (ISSN: 0022-3042)

ABSTRACT

In primary cultures of neurons from rat cerebral cortex and neostriatum, excitatory amino acids stimulate the translocation of protein kinase C (PKC) from the cytoplasm to the membrane. In the presence of a physiological concentration of Mg2+ in the extracellular medium, glutamate induces PKC translocation by binding to both N-methyl-D-aspartate (NMDA) and alpha-amino-3-hydroxy-5-methylisoxazolepropionic acid (AMPA) excitatory amino acid receptors. Quisqualate translocates the enzyme by stimulating primarily AMPA receptors and possibly metabotropic receptors. NMDA receptor-induced PKC translocation is sodium independent, whereas quisqualate receptor-induced PKC translocation is sodium dependent; none of the agonists is active in the absence of calcium from the extracellular medium. Muscimol does not modify excitatory amino acid stimulation; however, blockade of gamma-aminobutyric acid(A) receptors by bicuculline greatly enhances glutamate-induced PKC translocation. This enhancement is blocked by the NMDA receptor antagonist (+)-5-methyl-10,11-dihydro-5H-dibenzo[a,d]cyclohepten-5,10-imine hydrogen maleate (MK-801) and by tetrodotoxin.

This document is available from: http://directlinks.cc/files/muscimol/1649249.pdf

Author List: Vaccarino F M, Liljequist S, Tallman J F

Publication Types: Journal Article; Research Support, U.S. Gov't, P.H.S.

Substances mentioned in the article: Amino Acids; Glutamates; Oxadiazoles; Receptors, AMPA; Receptors, N-Methyl-D-Aspartate; Receptors, Neurotransmitter; Phorbol 12,13-Dibutyrate; Tetrodotoxin; Dizocilpine Maleate; Protein Kinase C; Bicuculline;

Mesh terms: Amino Acids/pharmacology; Animals; Animals, Newborn; Bicuculline/pharmacology; Cell Membrane/enzymology; Cells, Cultured; Cerebral Cortex/enzymology; Corpus Striatum/enzymology; Dizocilpine Maleate/pharmacology; Glutamates/pharmacology; Kinetics; Neurons/enzymology; Oxadiazoles/metabolism; Phorbol 12,13-Dibutyrate/metabolism; Protein Kinase C/metabolism; Rats; Rats, Inbred Strains; Receptors, AMPA; Receptors, N-Methyl-D-Aspartate/drug effects; Receptors, Neurotransmitter/drug effects; Tetrodotoxin/pharmacology;

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