Alternative splicing generates metabotropic glutamate receptors inducing different patterns of calcium release in Xenopus oocytes.

Article date: 1992/11/1

PubMed ID: 1649371

Journal name: Proceedings of the National Academy of Sciences of the United States of America (ISSN: 0027-8424)


A splice variant of the metabotropic glutamate receptor (mGluR) 1a, named mGluR1c, was isolated. Compared to mGluR1a, the predicted mGluR1c protein is 302 amino acids shorter at its C-terminal end. Despite this difference, mGluR1c activates phospholipase C in Xenopus oocytes with a pharmacological profile identical to that of mGluR1a. However, in contrast to the large fast transient responses induced by mGluR1a, mGluR1c receptors elicit a small more slowly generated long-lasting oscillatory current, suggesting that these two receptors do not generate the same pattern of Ca2+ release in Xenopus oocytes. In situ hybridization data show that mGluR1c mRNA is expressed at a lower level than the other splice variants of mGluR1. Some differences in the regional distribution of these transcripts were observed in the cerebellum, the olfactory bulb, and the striatum.

Author List: Pin J P, Waeber C, Prezeau L, Bockaert J, Heinemann S F

Publication Types: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.

Substances mentioned in the article: Glutamates; Neurotoxins; Oligodeoxyribonucleotides; Oligonucleotide Probes; Receptors, Glutamate; Cycloleucine; 1-amino-1,3-dicarboxycyclopentane; Ibotenic Acid; Aspartic Acid; Egtazic Acid; Kainic Acid; Calcium;

Mesh terms: Alternative Splicing; Amino Acid Sequence; Animals; Aspartic Acid/pharmacology; Base Sequence; Calcium/metabolism; Cycloleucine/analogs & derivatives; Egtazic Acid/pharmacology; Gene Library; Glutamates/pharmacology; Ibotenic Acid/pharmacology; Kainic Acid/pharmacology; Membrane Potentials/drug effects; Molecular Sequence Data; Neurotoxins/pharmacology; Oligodeoxyribonucleotides; Oligonucleotide Probes; Oocytes/metabolism; Polymerase Chain Reaction; Receptors, Glutamate/drug effects; Restriction Mapping; Xenopus;

Citations: - 12106464

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