Article date: 1991/9/9
PubMed ID: 1655526
Journal name: FEBS letters (ISSN: 0014-5793)
A cDNA of rat brain encoding the GABAA receptor alpha 4 subunit has been cloned. Recombinant receptors composed of alpha 4, beta 2 and gamma 2 subunit bind with high affinity the GABA agonist [3H]muscimol and the benzodiazepine 'alcohol antagonist' [3H]Ro 15-4513, but fail to bind benzodiazepine agonists. The alpha 4 subunit is expressed mainly in the thalamus, as assessed by in situ hybridization histochemistry, and may participate in a major population of thalamic GABAA receptors. The alpha 4 mRNA is found at lower levels in cortex and caudate putamen, and is rare in cerebellum.
Author List: Wisden W, Herb A, Wieland H, Keinänen K, Lüddens H, Seeburg P H
Publication Types: Journal Article; Research Support, Non-U.S. Gov't
Substances mentioned in the article: Affinity Labels; Azides; Gabra4 protein, rat; Macromolecular Substances; Oligodeoxyribonucleotides; RNA, Messenger; Receptors, GABA-A; Recombinant Proteins; Benzodiazepines; Muscimol; Ro 15-4513;
Mesh terms: Affinity Labels/metabolism; Amino Acid Sequence; Animals; Azides/metabolism; Base Sequence; Benzodiazepines/metabolism; Binding, Competitive; Brain/metabolism; Cloning, Molecular; Kinetics; Macromolecular Substances; Molecular Sequence Data; Muscimol/metabolism; Oligodeoxyribonucleotides; Organ Specificity; RNA, Messenger/analysis; Rats; Receptors, GABA-A/analysis; Recombinant Proteins/analysis;