1656338

Autoradiographic localisations of glutamatergic ligand binding sites in Xenopus brain.

Article date: 1991/8/5

PubMed ID: 1656338

Journal name: Neuroscience letters (ISSN: 0304-3940)

ABSTRACT

In the Xenopus central nervous system the binding sites for [3H]kainate and [3H]alpha-amino-3-hydroxy-5-methylisoxazolepropionate [( 3H]AMPA) are highly localised and the distributions of both ligands are largely coincident. The telencephalon was the most strongly labelled area with a relatively uniform distribution of binding sites. In addition, the infundibulum and the cerebellum were also heavily labelled. Areas containing a lower density of binding sites included the septum, the thalamus and the optic lobes. [3H]Kainate binding was potently inhibited by 1 microM AMPA in the presence of 0.1 M KSCN and [3H]AMPA binding was blocked by 1 microM kainate. These data are consistent with the hypothesis that kainate and AMPA bind to the same site on a single protein entity and that this unitary AMPA/KA binding protein may constitute the predominant type of excitatory amino acid receptor in the Xenopus brain.

Author List: Henley J M, Bond A, Barnard E A

Publication Types: Journal Article

Substances mentioned in the article: Receptors, Glutamate; Receptors, N-Methyl-D-Aspartate; Receptors, Neurotransmitter; Ibotenic Acid; N-Methylaspartate; alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid; Kainic Acid;

Mesh terms: Animals; Autoradiography; Binding Sites; Brain Chemistry; Ibotenic Acid/analogs & derivatives; Kainic Acid/metabolism; N-Methylaspartate/pharmacology; Receptors, Glutamate; Receptors, N-Methyl-D-Aspartate/analysis; Receptors, Neurotransmitter/analysis; Xenopus; alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid;

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