1664923

A 'macro' image analysis of fura-2 fluorescence to visualize the distribution of functional glutamate receptor subtypes in hippocampal slices.

Article date: 1991/11/1

PubMed ID: 1664923

Journal name: Neuroscience research (ISSN: 0168-0102)

ABSTRACT

The distribution of functional glutamate receptor subtypes was visualized as regional changes in intracellular Ca2+ concentration induced by administration of a specific agonist for each subtype. An image of the fura-2 fluorescence from a whole hippocampal slice was directly excited at 340 or 380 nm through an optical fibre and observed via a low-magnification objective lens (x4). A heterogeneous distribution of N-methyl-D-aspartate (NMDA) and non-NMDA subtypes was demonstrated by the ratio of fluorescence intensities before and after administration of each agonist. This 'macro' image analysis can reveal distributions of multiple functional receptors in a living brain slice.

Author List: Kudo Y, Nakamura T, Ito E

Publication Types: Journal Article; Research Support, Non-U.S. Gov't

Substances mentioned in the article: Receptors, Glutamate; Receptors, Neurotransmitter; Ibotenic Acid; alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid; Calcium; Fura-2;

Mesh terms: Animals; Calcium/metabolism; Fluorescence; Fura-2; Hippocampus/anatomy & histology; Ibotenic Acid/analogs & derivatives; Image Processing, Computer-Assisted; In Vitro Techniques; Rats; Receptors, Glutamate; Receptors, Neurotransmitter/drug effects; alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid;

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