Reduction in bioelectric GABA and NMDA responses in organotypic neocortical explants by chronic elevation of potassium.

Article date: 1991/9/30

PubMed ID: 1665212

Journal name: Neuroscience letters (ISSN: 0304-3940)


Long-term cultures of organotypic neonatal rat neocortex slices were maintained in a serum-free medium supplemented with 25 mM potassium. Such cultures continue to be bioelectrically silent upon return to a 5 mM potassium medium. The absence of responses to pressure ejected gamma-aminobutyric acid (GABA) and N-methyl-D-aspartate (NMDA) from neurons in treated explants suggests that one consequence of chronic depolarization is a reduction in the density of postsynaptic transmitter receptors. [3H]Muscimol binding to neocortical membrane preparations shows a large reduction in the binding of this agonist to GABAA receptors. These data show that the quantitative expression of at least one neurotransmitter receptor, the GABAA receptor, relies on voltage-dependent activity in developing neocortical neurons in vitro.

This document is available from: http://directlinks.cc/files/muscimol/1665212.pdf

Author List: Baker R E, Ruijter J M, Walden J, Bingmann D, Straub H, Speckmann E J

Publication Types: Journal Article

Substances mentioned in the article: Receptors, GABA-A; Muscimol; gamma-Aminobutyric Acid; N-Methylaspartate; Dizocilpine Maleate; Potassium;

Mesh terms: Action Potentials/drug effects; Animals; Animals, Newborn; Cell Membrane/metabolism; Cerebral Cortex/drug effects; Dizocilpine Maleate/pharmacology; Membrane Potentials/drug effects; Muscimol/metabolism; N-Methylaspartate/pharmacology; Neurons/drug effects; Organ Culture Techniques; Potassium/pharmacology; Rats; Receptors, GABA-A/metabolism; gamma-Aminobutyric Acid/pharmacology;

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