Intercellular action of nitric oxide in adult rat cerebellar slices.

Article date: 1991/11/1

PubMed ID: 1687356

Journal name: Neuroreport (ISSN: 0959-4965)


Nitric oxide (NO) is a novel messenger molecule that is produced following glutamate receptor activation and which stimulates cyclic GMP (cGMP) formation. To determine if the mode of action of NO is predominantly intra- or inter-cellular in intact brain tissue, we tested the ability of haemoglobin (Hb), a NO-binding protein that remains extracellular, to inhibit cGMP accumulation induced by glutamate receptor agonists in adult rat cerebellar slices. Responses to agonists acting selectively on N-methyl-D-aspartate (NMDA) and non-NMDA glutamate receptors were inhibited by Hb (IC50 approximately 20 microM) suggesting that NO functions predominantly as a mediator of communication between cells. The effects of NO-donating drugs (e.g. nitroprusside) were also inhibited by Hb, implying that they yield NO extracellularly.

Author List: Southam E, Garthwaite J

Publication Types: Journal Article; Research Support, Non-U.S. Gov't

Substances mentioned in the article: Glutamates; Hemoglobins; Hydroxylamines; Receptors, Glutamate; Receptors, N-Methyl-D-Aspartate; Receptors, Neurotransmitter; Nitroprusside; Ibotenic Acid; Hydroxylamine; Nitric Oxide; Glutamic Acid; linsidomine; N-Methylaspartate; alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid; S-Nitroso-N-Acetylpenicillamine; Molsidomine; Penicillamine; Cyclic GMP; Kainic Acid;

Mesh terms: Animals; Cerebellum/drug effects; Cyclic GMP/metabolism; Drug Interactions; Glutamates/pharmacology; Glutamic Acid; Hemoglobins/pharmacology; Hydroxylamine; Hydroxylamines/pharmacology; Ibotenic Acid/analogs & derivatives; Kainic Acid/pharmacology; Molsidomine/analogs & derivatives; N-Methylaspartate/pharmacology; Nitric Oxide/pharmacology; Nitroprusside/pharmacology; Penicillamine/analogs & derivatives; Rats; Receptors, Glutamate; Receptors, N-Methyl-D-Aspartate/drug effects; Receptors, Neurotransmitter/drug effects; S-Nitroso-N-Acetylpenicillamine; alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid;

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