Participation of postsynaptic PKC in cerebellar long-term depression in culture.

Article date: 1991/12/13

PubMed ID: 1721243

Journal name: Science (New York, N.Y.) (ISSN: 0036-8075)


Long-term depression (LTD) in the intact cerebellum is a decrease in the efficacy of the parallel fiber-Purkinje neuron synapse induced by coactivation of climbing fiber and parallel fiber inputs. In cultured Purkinje neurons, a similar depression can be induced by iontophoretic glutamate pulses and Purkinje neuron depolarization. This form of LTD is expressed as a depression of alpha-amino-3-hydroxy-5-methyl-4- isoxazole-propionic acid (AMPA)-mediated current, and its induction is dependent on activation of metabotropic quisqualate receptors. The effect of inhibitors of protein kinase C (PKC) on LTD induction was studied. Inhibitors of PKC blocked LTD induction, while phorbol-12,13-diacetate (PDA), a PKC activator, mimicked LTD. These results suggest that PKC activation is necessary for the induction of cerebellar LTD.

Author List: Linden D J, Connor J A

Publication Types: Journal Article

Substances mentioned in the article: Indoles; Naphthalenes; Phorbol Esters; Polycyclic Compounds; Receptors, AMPA; Receptors, Neurotransmitter; Ibotenic Acid; alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid; Quisqualic Acid; Protein Kinase C; calphostin C; Calcium; Ro 31-8220;

Mesh terms: Animals; Calcium/physiology; Cells, Cultured; Cerebellum/physiology; Enzyme Activation/drug effects; Ibotenic Acid/analogs & derivatives; In Vitro Techniques; Indoles; Mice; Naphthalenes; Phorbol Esters/pharmacology; Polycyclic Compounds/pharmacology; Protein Kinase C/antagonists & inhibitors; Purkinje Cells/physiology; Quisqualic Acid/pharmacology; Receptors, AMPA; Receptors, Neurotransmitter/physiology; Synaptic Membranes/physiology; alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid;

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