Article date: 1991/12/16
PubMed ID: 1721809
Journal name: Biochemical and biophysical research communications (ISSN: 0006-291X)
Using a baculovirus expression vector system, the alpha 1 subunit of the mouse AMPA (alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate)-selective glutamate receptor channel was expressed in insect Spdoptera frugiperda cells. Binding studies using [3H]AMPA showed that insect cells infected with the recombinant virus expressed approximately 1.8 x 10(5) binding sites per cell on their surface. The ligand binding characteristics of the receptors expressed in insect cells were examined. The baculovirus-insect cell expression system affords high-efficiency expression of the receptor in sufficient amounts to permit structural and functional analyses.
Author List: Kawamoto S, Onishi H, Hattori S, Miyagi Y, Amaya Y, Mishina M, Okuda K
Publication Types: Journal Article
Substances mentioned in the article: DNA, Recombinant; Glutamates; Ion Channels; Receptors, Glutamate; Receptors, Neurotransmitter; Ibotenic Acid; Glutamic Acid; alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid; Quisqualic Acid; Kainic Acid;
Mesh terms: Animals; Baculoviridae/genetics; DNA, Recombinant; Gene Expression; Genetic Vectors; Glutamates/metabolism; Glutamic Acid; Ibotenic Acid/analogs & derivatives; Insecta/genetics; Ion Channels/metabolism; Kainic Acid/metabolism; Kinetics; Mice; Quisqualic Acid/pharmacology; Receptors, Glutamate; Receptors, Neurotransmitter/drug effects; Transfection; alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid;