Article date: 1991/11/1
PubMed ID: 1725727
Journal name: Neuroreport (ISSN: 0959-4965)
We have studied by in situ hybridization the mRNA expression of the pp60c-src proto-oncogene in grafts of foetal striatal neurons implanted into the ibotenic acid lesioned adult rat neostriatum. Animals were studied at 5, 10, 15, 30 days and 4 months after transplantation. Using 35S-labelled specific oligonucleotide probes, high levels of mRNA for pp60c-src+ but not pp60c-src- were found in the normal embryonic striatum and in striatal grafts up to 1 month after implantation; no hybridization signal was obtained in the normal adult striatum or in 4-month old grafts. The data suggest that pp60c-src+ is highly expressed in developing, but not mature, neurons and may play a fundamental role in the maturation and differentiation of grafted embryonic striatal neurons.
Author List: Mayer E, Heavens R P, Dunnett S B, Sirinathsinghji D J
Publication Types: Journal Article
Substances mentioned in the article: Nerve Tissue Proteins; RNA, Messenger; Ibotenic Acid; Proto-Oncogene Proteins pp60(c-src);
Mesh terms: Animals; Brain Tissue Transplantation; Corpus Striatum/drug effects; Female; Fetal Tissue Transplantation; Gene Expression Regulation, Enzymologic; Genes, src; Ibotenic Acid/toxicity; Nerve Tissue Proteins/biosynthesis; Neurons/drug effects; Nucleic Acid Hybridization; Proto-Oncogene Proteins pp60(c-src)/biosynthesis; RNA, Messenger/biosynthesis; Rats; Rats, Inbred Strains;