Interaction of 5-bromowillardiine with non-NMDA receptors expressed in Xenopus laevis oocytes injected with chick brain mRNA.

Article date: 1991/1/2

PubMed ID: 1850505

Journal name: Neuroscience letters (ISSN: 0304-3940)


Excitatory amino acid (EAA) receptors were studied in Xenopus laevis oocytes microinjected with 1-day-old chick brain mRNA using a two-electrode voltage clamp technique. EAA agonists could be differentially characterised according to their current/voltage (I/V) relationships; I/V plots to kainate (KA) and domoate (Dom) inwardly rectified at positive membrane potentials whereas quisqualate (QA), alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) and L-glutamate (L-Glu) produced linear I/V plots. Interestingly, the non-NMDA (N-methyl-D-aspartate) agonist, 5-bromowillardiine (BrW), displayed properties similar to KA and Dom. It is proposed that BrW acts as a KA-like agonist on chick EAA receptors expressed in Xenopus oocytes.

Author List: Bowie D, Smart T G

Publication Types: Journal Article; Research Support, Non-U.S. Gov't

Substances mentioned in the article: Neuromuscular Depolarizing Agents; RNA, Messenger; Receptors, Dopamine; Receptors, Kainic Acid; Receptors, N-Methyl-D-Aspartate; Receptors, Neurotransmitter; 5-bromowillardiine; Ibotenic Acid; alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid; Quisqualic Acid; domoic acid; Alanine; Kainic Acid;

Mesh terms: Alanine/analogs & derivatives; Animals; Brain; Chickens; Female; Ibotenic Acid/analogs & derivatives; Injections; Kainic Acid/analogs & derivatives; Membrane Potentials/drug effects; Neuromuscular Depolarizing Agents; Oocytes/metabolism; Quisqualic Acid; RNA, Messenger/administration & dosage; Receptors, Dopamine/drug effects; Receptors, Kainic Acid; Receptors, N-Methyl-D-Aspartate/physiology; Receptors, Neurotransmitter/metabolism; Xenopus laevis/metabolism; alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid;

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