Article date: 1991/6/1
PubMed ID: 1851208
Journal name: Journal of neurochemistry (ISSN: 0022-3042)
The bovine gamma-aminobutyric acidA/benzodiazepine receptor complex has been purified by a novel immunoaffinity chromatography method on immobilized monoclonal antibody 62-3G1. Immunopurification of the complex was achieved in a single step with an improved yield over affinity chromatography on the benzodiazepine Ro 7-1986/1. High-resolution sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of the immunoaffinity-purified receptor revealed three major peptide bands of 51,000, 55,000, and 57,000 Mr which were also present in the Ro 7-1986/1 affinity-purified receptor. Peptide mapping, immunoblotting with subunit specific antibodies, and photoaffinity labeling with [3H]flunitrazepam and [3H]muscimol have been used for the identification of receptor subunits, including several which comigrated in a single band in SDS-PAGE.
Author List: Park D, Vitorica J, Tous G, de Blas A L
Publication Types: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.
Substances mentioned in the article: Affinity Labels; Antibodies, Monoclonal; Immunosorbents; Ligands; Peptides; Receptors, GABA-A;
Mesh terms: Affinity Labels; Animals; Antibodies, Monoclonal; Cattle; Cerebral Cortex/metabolism; Chromatography, Affinity; Immunosorbents; Ligands; Peptide Mapping; Peptides/metabolism; Receptors, GABA-A/isolation & purification;