Article date: 1991/2/1
PubMed ID: 1851978
Journal name: Neuroscience research (ISSN: 0168-0102)
Pharmacological properties of metabotropic glutamate receptors were studied in Xenopus oocytes injected with rat brain mRNA. trans-1-Amino-cyclopentyl-1,3-dicarboxylic acid (t-ACPD), a conformationally restricted analog of glutamate, induced oscillatory inward currents in mRNA-injected oocytes. These t-ACPD responses showed several characteristics identical to those of the other metabotropic responses including the metabotropic glutamate responses stimulated by quisqualate. D,L-2-Amino-3-phophonopropionate (D,L-AP3) effectively suppressed the t-ACPD and ibotenate responses. However, quisqualate responses were not affected substantially by D,L-AP3. These findings suggest that the metabotropic glutamate receptors in the oocytes may be classified into at least two subtypes according to their pharmacological properties: one preferentially activated by quisqualate and insensitive to AP3, and the other activated by t-ACPD and ibotenate and antagonized by AP3.
Author List: Tanabe S, Ito I, Sugiyama H
Publication Types: Journal Article
Substances mentioned in the article: RNA, Messenger; Receptors, Glutamate; Receptors, Neurotransmitter; 2-amino-3-phosphonopropionic acid; Cycloleucine; 1-amino-1,3-dicarboxycyclopentane; Ibotenic Acid; Quisqualic Acid; GTP-Binding Proteins; Alanine;
Mesh terms: Alanine/analogs & derivatives; Animals; Brain Chemistry; Cycloleucine/analogs & derivatives; GTP-Binding Proteins/pharmacology; Ibotenic Acid/pharmacology; Oocytes/drug effects; Quisqualic Acid/pharmacology; RNA, Messenger/pharmacology; Rats; Receptors, Glutamate; Receptors, Neurotransmitter/drug effects; Xenopus/metabolism;