Article date: 1991/3/1
PubMed ID: 1856884
Journal name: Journal of neuroscience research (ISSN: 0360-4012)
The present investigation was designed to determine the effect of lesions localized to the nucleus basalis/substantia innominata (NB) on resting and cholinergically activated regional cerebral cortical blood flow (rCBF). Ibotenic acid (10 micrograms) was infused locally at 1 mm caudal to bregma, 3 mm lateral to the midline, and 8 mm below the cortical surface. Effectiveness of lesions was demonstrated by histological verification of lesion sites and determination of choline acetyltransferase activity in cerebral cortex homogenates. rCBF was measured with the autoradiographic iodo-14C-antipyrine technique. Resting rCBF was similar in the hemisphere that received the NB lesion and in the contralateral (intact) side in all regions examined. Physostigmine intravenous infusion (3.3 micrograms.kg-1.min-1) enhanced rCBF in frontal, parietal, occipital, and temporal cortex. The increase was symmetrical, however, indicating inability of NB lesion to affect this phenomenon. It is concluded that the cortical cholinergic afferents originating in the NB are not involved in the control of rCBF.
Author List: Scremin O U, Torres C, Scremin A M, O'Neal M, Heuser D, Blisard K S
Publication Types: Journal Article; Research Support, U.S. Gov't, Non-P.H.S.
Substances mentioned in the article: Biomarkers; Ibotenic Acid; Physostigmine; Choline O-Acetyltransferase; Acetylcholine;
Mesh terms: Acetylcholine/physiology; Afferent Pathways/physiology; Animals; Biomarkers; Cerebral Cortex/blood supply; Cerebrovascular Circulation/physiology; Choline O-Acetyltransferase/analysis; Cholinergic Fibers/drug effects; Ibotenic Acid/toxicity; Male; Olivary Nucleus/drug effects; Physostigmine/pharmacology; Rats; Rats, Inbred Strains; Substantia Innominata/drug effects;