196200

gamma-Aminobutyric acid receptors visualized in spinal cord cultures by [3H]muscimol autoradiography.

Article date: 1978/12/1

PubMed ID: 196200

Journal name: Proceedings of the National Academy of Sciences of the United States of America (ISSN: 0027-8424)

ABSTRACT

gamma-Aminobutyric acid (GABA) receptors were visualized in mouse spinal cord explant cultures by [3H]muscimol autoradiography over certain small neurons of the dorsal horn grey matter, in the interneuronal neuropil of dorsal and ventral horns, and (rarely) in small clusters on processes of anterior horn cells. Specificity was indicated in control experiments by inhibition of binding by [3H]muscimol after pretreatment with GABA or a GABA analogue, 4,5,6,7-tetrahydroisoxazolo[5,4-c]pyridin-3-ol (THIP, isoxazole 16), or with receptor antagonists bicuculline and picrotoxin. Unlabeled muscimol exchanged effectively with [3H]muscimol and produced autoradiographic label in locations indistinguishable from those found in experiments with [3H]muscimol alone. Pretreatment with the GABA uptake and transport inhibitors (-)-nipecotic acid and guvacine did not affect binding with [3H]muscimol. These experiments indicate that explant culture systems can be used for demonstration of functional receptors.

Author List: Chan-Palay V, Yonezawa T, Yoshida S, Palay S

Publication Types: Journal Article

Substances mentioned in the article: Receptors, Neurotransmitter; Muscimol; gamma-Aminobutyric Acid;

Mesh terms: Animals; Autoradiography; Cells, Cultured; Mice; Muscimol/metabolism; Receptors, Neurotransmitter/metabolism; Spinal Cord/metabolism; gamma-Aminobutyric Acid/metabolism;

Citations: - 14303905

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