1973276

L-[3H]glutamate labels the metabotropic excitatory amino acid receptor in rodent brain.

Article date: 1990/5/18

PubMed ID: 1973276

Journal name: Neuroscience letters (ISSN: 0304-3940)

ABSTRACT

A quantitative autoradiographic assay for a novel L-[3H]glutamate binding site in rodent brain has been developed. Binding to this site was distinguished by its high affinity for quisqualate (QA), ibotenate, glutamate and trans-1-amino-cyclopentyl-1,3-dicarboxylic acid (trans-ACPD), but low affinity for [RS]-alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA), kainate and N-methyl-D-aspartate (NMDA). 'AMPA-insensitive, QA-sensitive [3H]glutamate binding' (AiQsGB) had a heterogeneous distribution in rat brain with high levels observed in molecular layer of cerebellum, striatum, and lateral septum. AiQsGB was reduced in molecular layer of cerebellum in mice lacking Purkinje cells. AiQsGB appears to represent binding to the 'metabotropic' neuronal excitatory amino acid receptor linked to phosphoinositide metabolism.

Author List: Cha J H, Makowiec R L, Penney J B, Young A B

Publication Types: Journal Article; Research Support, U.S. Gov't, P.H.S.

Substances mentioned in the article: Glutamates; Neurotransmitter Agents; Oxadiazoles; Receptors, Amino Acid; Receptors, Cell Surface; Ibotenic Acid; Glutamic Acid; Quisqualic Acid;

Mesh terms: Animals; Brain/metabolism; Glutamates/metabolism; Glutamic Acid; Ibotenic Acid/metabolism; Neurotransmitter Agents/metabolism; Oxadiazoles/metabolism; Quisqualic Acid; Rats; Receptors, Amino Acid; Receptors, Cell Surface/metabolism;

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