Article date: 1990/8/1
PubMed ID: 2115071
Journal name: Journal of neurochemistry (ISSN: 0022-3042)
The locus coeruleus (LC) of the rat was lesioned by microinjection of selective neurotoxins into the brainstem. 6-Hydroxydopamine (6-OHDA), 3 micrograms/microliter, given unilaterally at two sites 0.6 mm apart on the rostro-caudal axis of the LC, was used to lesion catecholamine-containing neuronal elements. Ibotenic acid, 2.5 micrograms/0.5 microliters, administered similarly was used to lesion nerve cell bodies. Two weeks after administration of the neurotoxin, lesion efficacy was determined based on the norepinephrine content of the cerebral cortex ipsi- and contralateral to the lesion. 6-OHDA lesions of the LC caused a 46% reduction in ipsilateral cortical norepinephrine and a 60% reduction in specific 125I-[Sar1, Ile8]-angiotensin II (125I-SIAII) binding in the LC. Ibotenic acid lesions of the LC caused a 73% reduction in ipsilateral cortical norepinephrine and a 81% reduction in specific 125I-SIAII binding in the LC. These results indicate that AII receptor binding sites in the LC are localized on noradrenergic nerve cell bodies or their dendritic and axonal ramifications within the LC.
Author List: Rowe B P, Kalivas P W, Speth R C
Publication Types: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.
Substances mentioned in the article: Hydroxydopamines; Receptors, Angiotensin; Angiotensin II; Ibotenic Acid; Oxidopamine; 1-Sarcosine-8-Isoleucine Angiotensin II; Norepinephrine;
Mesh terms: 1-Sarcosine-8-Isoleucine Angiotensin II/metabolism; Angiotensin II/metabolism; Animals; Autoradiography; Cerebral Cortex/metabolism; Hydroxydopamines/pharmacology; Ibotenic Acid/pharmacology; Locus Coeruleus/drug effects; Male; Neurons/metabolism; Norepinephrine/metabolism; Oxidopamine; Rats; Rats, Inbred Strains; Receptors, Angiotensin/metabolism;