Article date: 1990/2/5
PubMed ID: 2156190
Journal name: Neuroscience letters (ISSN: 0304-3940)
In striatal neurons in primary culture quisqualate potently stimulated the formation of inositol phosphates via a metabotropic receptor we recently termed Qp in order to distinguish it from the classical ionotropic quisqualate receptor termed Qi. Here we show that 10 microM of quisqualate activated in a rapid and transient manner protein kinase C as assessed by its translocation from the cytosolic to the membrane fraction. As 10 microM alpha-amino-3-hydroxy-5-methylisoxazole-4-propionic acid (AMPA), the Qi specific agonist, was without effect, this translocation was most probably mediated by the Qp receptor. Phorbol 12,13-dibutyrate blocked in a dose-dependent manner the Qp receptor-induced inositol phosphate formation (IC50 = 2 +/- 0.4 nM). The inactive ester 4 alpha-phorbol-12,13-didecanoate was without effect. Very low concentrations of staurosporine completely reversed the phorbol 12,13-dibutyrate-induced blockade (IC50 = 2.2 +/- 1.3 nM). It can therefore be concluded that the Qp receptor is able to activate protein kinase C and that the activity of this metabotropic receptor is regulated by protein kinase C.
Author List: Manzoni O J, Finiels-Marlier F, Sassetti I, Blockaert J, le Peuch C, Sladeczek F A
Publication Types: Journal Article; Research Support, Non-U.S. Gov't
Substances mentioned in the article: Alkaloids; Receptors, AMPA; Receptors, Neurotransmitter; Ibotenic Acid; Phorbol 12,13-Dibutyrate; alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid; Protein Kinase C; Staurosporine;
Mesh terms: Alkaloids/pharmacology; Animals; Corpus Striatum/drug effects; Cytosol/enzymology; Ibotenic Acid/analogs & derivatives; Phorbol 12,13-Dibutyrate/pharmacology; Protein Kinase C/metabolism; Receptors, AMPA; Receptors, Neurotransmitter/drug effects; Staurosporine; alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid;