Chronic ethanol and pentobarbital administration in the rat: effects on GABAA receptor function and expression in brain.

Article date: 1990/5/1

PubMed ID: 2158791

Journal name: Alcohol (Fayetteville, N.Y.) (ISSN: 0741-8329)


Chronic exposure of rats to ethanol significantly decrease GABAA receptor-mediated 36Cl- uptake in cerebral cortical synaptoneurosomes. Muscimol and pentobarbital stimulation as well as ethanol enhancement of muscimol-stimulated 36Cl- flux are significantly decreased following chronic ethanol inhalation. Repeated pentobarbital administration has a similar effect on muscimol and pentobarbital-stimulated 36Cl- uptake in cerebral cortical synaptoneurosomes. We have postulated that these adaptive response may be associated with an alteration of GABAA receptor gene expression. Chronic ethanol exposure resulted in a significant reduction in the levels of GABAA receptor alpha-subunit mRNA's. The most abundant mRNA species in the rat cerebral cortex were reduced 40-50% (4.4 Kb mRNA, 43%, 4.8 Kb mRNA, 47%). beta-Actin mRNA and poly(A)+ RNA levels were not significantly reduced following chronic ethanol exposure. Repeated pentobarbital administration had no effect on the level of the 4.4 and 4.8 Kb transcripts of alpha-subunit mRNAs in rat cerebral cortex. These data suggest that chronic ethanol exposure alters the level of mRNA's coding for the alpha-subunit of the GABAA receptor. This decrease may reflect an alteration of mRNA processing in the cell or an alteration in GABAA receptor gene expression.

This document is available from: http://directlinks.cc/files/muscimol/2158791.pdf

Author List: Morrow A L, Montpied P, Lingford-Hughes A, Paul S M

Publication Types: Journal Article

Substances mentioned in the article: Receptors, GABA-A; Ethanol; Pentobarbital;

Mesh terms: Animals; Brain Chemistry/drug effects; Ethanol/pharmacology; Male; Pentobarbital/pharmacology; Rats; Rats, Inbred Strains; Receptors, GABA-A/drug effects;

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