2160394

Quantitative analysis of gamma-aminobutyric acid (GABA) receptors of Purkinje cell layer from rat cerebellar slices.

Article date: 1990/1/1

PubMed ID: 2160394

Journal name: General pharmacology (ISSN: 0306-3623)

ABSTRACT

1. Extracellular recordings were made from Purkinje cell layer of rat cerebellar slices. Cell activity was sensitive to both magnesium and manganese ions. 2. Glutamic and aspartic acids both excited cell activity while gamma-aminobutyric acid (GABA), muscimol, taurine, beta-alanine and delta-aminolaevulinic acid all inhibited activity. 3. The sensitivity to GABA varied with depth while no such effect was observed with muscimol. 4. Bicuculline methiodide, picrotoxin and pitrazepin blocked the action of muscimol with pA2 values of 5.92, 5.97 and 5.71 respectively. 5. The benzodiazepines flurazepam and RU 32007 both potentiated the GABA inhibition and this potentiation was blocked by Ro 15-1788, a benzodiazepine antagonist.

Author List: Hussain S, Bagust J, Gardner C R, Ward R A, Walker R J

Publication Types: Journal Article

Substances mentioned in the article: Glutamates; Receptors, GABA-A; Muscimol; Aspartic Acid; gamma-Aminobutyric Acid;

Mesh terms: Animals; Aspartic Acid/metabolism; Cerebellum/cytology; Drug Interactions; Female; Glutamates/metabolism; In Vitro Techniques; Muscimol/antagonists & inhibitors; Neurons/drug effects; Purkinje Cells/metabolism; Rats; Receptors, GABA-A/drug effects; gamma-Aminobutyric Acid/pharmacology;

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