2165430

Electrophysiological evidence for the existence of GABAA receptors in cultured frog melanotrophs.

Article date: 1990/5/28

PubMed ID: 2165430

Journal name: Brain research (ISSN: 0006-8993)

ABSTRACT

The neurotransmitter GABA exerts a biphasic effect on alpha-melanocyte-stimulating hormone (alpha-MSH) secretion from pars intermedia cells: GABA induces a rapid and transient stimulation followed by a sustained inhibition of alpha-MSH release. In the present study, we have investigated the effect of GABA on the electrophysiological properties of frog melanotrophs in primary culture using the patch-clamp technique in the whole cell configuration. In all cells tested, GABA stimulated an inward current and induced depolarization. A transient period of intense firing was consistently observed at the onset of GABA administration. During the depolarization phase, the membrane potential reached a plateau corresponding to the Cl- equilibrium potential. When repeated hyperpolarizing pulses were applied, an increase of membrane conductance was observed throughout the response evoked by GABA. The effect of GABA was abolished by the chloride channel blocker picrotoxin, and by antagonists of GABAA receptors (bicuculline and SR 95531). The depolarizing action of GABA was mimicked by muscimol, an agonist of GABAA receptors. Taken together, our results indicate that the rapid and transient stimulation of alpha-MSH release induced by GABA can be accounted for by activation of a chloride conductance which causes membrane depolarization. These data support the notion that the transient stimulation of alpha-MSH secretion induced by GABA can be accounted for by membrane depolarization which provokes activation of voltage-operated calcium channels. Since no evidence was found for GABA-induced hyperpolarization, the intracellular mechanisms leading to the strong inhibitory effect of GABA on alpha-MSH secretion remain to be elucidated.

This document is available from: http://directlinks.cc/files/muscimol/2165430.pdf

Author List: Louiset E, van de Put F H, Tonon M C, Basille C, Jenks B G, Vaudry H, Cazin L

Publication Types: Journal Article; Research Support, Non-U.S. Gov't

Substances mentioned in the article: Chloride Channels; Chlorides; Membrane Proteins; Receptors, GABA-A; Picrotoxin; gamma-Aminobutyric Acid; alpha-MSH;

Mesh terms: Animals; Cells, Cultured; Chloride Channels; Chlorides/physiology; Male; Membrane Potentials/drug effects; Membrane Proteins/physiology; Picrotoxin/pharmacology; Pituitary Gland, Posterior/cytology; Rana ridibunda/metabolism; Ranidae/physiology; Receptors, GABA-A/drug effects; alpha-MSH/metabolism; gamma-Aminobutyric Acid/pharmacology;

2165430.txt · Last modified: 2018/11/22 21:16 (external edit)