Article date: 1990/8/3
PubMed ID: 2166337
Journal name: Science (New York, N.Y.) (ISSN: 0036-8075)
Four cloned cDNAs encoding 900-amino acid putative glutamate receptors with approximately 70 percent sequence identity were isolated from a rat brain cDNA library. In situ hybridization revealed differential expression patterns of the cognate mRNAs throughout the brain. Functional expression of the cDNAs in cultured mammalian cells generated receptors displaying alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA)-selective binding pharmacology (AMPA = quisqualate greater than glutamate greater than kainate) as well as cation channels gated by glutamate, AMPA, and kainate and blocked by 6,7-dinitroquinoxaline-2,3-dione (CNQX).
Author List: Keinänen K, Wisden W, Sommer B, Werner P, Herb A, Verdoorn T A, Sakmann B, Seeburg P H
Publication Types: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't
Substances mentioned in the article: Glutamates; Oligonucleotide Probes; Oxadiazoles; Oxazoles; RNA, Messenger; Receptors, Glutamate; Receptors, Neurotransmitter; Ibotenic Acid; alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid; Quisqualic Acid; Kainic Acid;
Mesh terms: Amino Acid Sequence; Animals; Base Sequence; Brain/metabolism; Glutamates/metabolism; Ibotenic Acid/analogs & derivatives; Kainic Acid/pharmacology; Kinetics; Molecular Sequence Data; Multigene Family; Oligonucleotide Probes; Organ Specificity; Oxadiazoles/pharmacology; Oxazoles/pharmacology; Quisqualic Acid; RNA, Messenger/analysis; Rats; Receptors, Glutamate; Receptors, Neurotransmitter/drug effects; Sequence Homology, Nucleic Acid; alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid;