Modulation of DL-alpha-amino-3-hydroxy-5-methylisoxazole-4-propionate (AMPA)/quisqualate receptors by phospholipase A2 treatment.

Article date: 1990/10/16

PubMed ID: 2177177

Journal name: Neuroscience letters (ISSN: 0304-3940)


The expression of long-term potentiation (LTP) in area CA1 of hippocampus has been proposed to result from an increased sensitivity of the AMPA/quisqualate receptors. We have investigated the binding properties of excitatory amino acid receptors in phospholipase A2 (PLA2)-treated rat brain membranes. PLA2 from bee venom produced a significant increase in the binding of [3H]-AMPA ([3H]-amino-3-hydroxy-5-methylisoxazole-4- propionate), a ligand for the AMPA/quisqualate receptor. Analysis of the saturation kinetics revealed that PLA2 treatment increased the affinity of the AMPA/quisqualate receptor without changing the maximum number of sites. In contrast, PLA2 treatment did not detectably modify the binding of [3H]-kainate to the kainate receptor and of [3H]-glutamate and [3H]-glycine to the NMDA (N-methyl-D-aspartate) receptor complex. These finding suggest that phospholipase A2 may regulate the AMPA/quisqualate receptor and could play an important role in the development of LTP.

Author List: Massicotte G, Baudry M

Publication Types: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.

Substances mentioned in the article: Glutamates; Receptors, AMPA; Receptors, Neurotransmitter; Tritium; Ibotenic Acid; alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid; Phospholipases A; Phospholipases A2; Kainic Acid; Glycine;

Mesh terms: Animals; Glutamates/metabolism; Glycine/metabolism; Hippocampus/drug effects; Ibotenic Acid/analogs & derivatives; Kainic Acid/metabolism; Male; Phospholipases A/pharmacology; Phospholipases A2; Radioligand Assay; Rats; Rats, Inbred Strains; Receptors, AMPA; Receptors, Neurotransmitter/drug effects; Synaptic Membranes/drug effects; Tritium; alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid;

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