218679

An analysis of [3H]gamma-aminobutyric acid (GABA) binding in the human brain.

Article date: 1979/3/9

PubMed ID: 218679

Journal name: Brain research (ISSN: 0006-8993)

ABSTRACT

The binding of [3H]GABA to membranes prepared from human brains obtained post morten was examined. This binding was independent of patient sex, age (16โ€“80 years), postmortem interval (4โ€“33 h) or storage time when frozen (0-64 months). In preparations from cerebellar cortex various compounds displaced [3H]GABA binding with the following order of potency: muscimol greater than 3-aminopropanesulfonic acid greater than GABA greater than imidazoleacet acid greater than delta-amino-n-valeric acid greater than 3-hydroxyGABA greater than bicuculline. Other compounds active 'in vitro' included strychnine, homocarnosine and some (e.g. clozapine, thioridazine, pimozide) but not all (chlorpromazine, haloperiodol) neuroleptics. Compounds inactive 'in vitro' included aminooxyacetic acid, baclofen, picrotoxin, anticholinergics, metrazole, anticonvulsants and naloxone. Triton X-100 augmented the [3H]GABA binding (25 nM) by about 10โ€“20-fold in most brain regions. [3H]GABA binding (IC50) was altered in Huntington's chorea and Reye's syndrome, but not in schizophrenics (4-neuroleptic-treated patients) or sudden infant death syndrome. The data presented strongly support the proposal that the measurement of [3H]GABA binding in postmortem human brain offers a reflection of the state of the physiologically relevant GABA receptor.

This document is available from: http://directlinks.cc/files/muscimol/218679.pdf

Author List: Lloyd K G, Dreksler S

Publication Types: Journal Article

Substances mentioned in the article: Receptors, Neurotransmitter; gamma-Aminobutyric Acid;

Mesh terms: Adolescent; Adult; Aged; Binding Sites/drug effects; Binding, Competitive/drug effects; Brain/metabolism; Caudate Nucleus/metabolism; Cerebellar Cortex/metabolism; Female; Globus Pallidus/metabolism; Hippocampus/metabolism; Humans; Huntington Disease/metabolism; Male; Middle Aged; Putamen/metabolism; Receptors, Neurotransmitter/drug effects; Reye Syndrome/metabolism; gamma-Aminobutyric Acid/metabolism;

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