218679

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218679 [2018/11/22 21:16] (current)
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 +====== An analysis of [3H]gamma-aminobutyric acid (GABA) binding in the human brain.======
 +
 +** Article date:** 1979/3/9
 +~~META:date created = 1979-03-09~~
 +
 +** PubMed ID: 218679 **
 +
 +** Journal name: Brain research (ISSN: 0006-8993) **
 +
 +** ABSTRACT **
 +
 +The binding of [3H]GABA to membranes prepared from human brains obtained post morten was examined. This binding was independent of patient sex, age (16--80 years), postmortem interval (4--33 h) or storage time when frozen (0-64 months). In preparations from cerebellar cortex various compounds displaced [3H]GABA binding with the following order of potency: muscimol greater than 3-aminopropanesulfonic acid greater than GABA greater than imidazoleacet acid greater than delta-amino-n-valeric acid greater than 3-hydroxyGABA greater than bicuculline. Other compounds active 'in vitro' included strychnine, homocarnosine and some (e.g. clozapine, thioridazine,​ pimozide) but not all (chlorpromazine,​ haloperiodol) neuroleptics. Compounds inactive 'in vitro' included aminooxyacetic acid, baclofen, picrotoxin, anticholinergics,​ metrazole, anticonvulsants and naloxone. Triton X-100 augmented the [3H]GABA binding (25 nM) by about 10--20-fold in most brain regions. [3H]GABA binding (IC50) was altered in Huntington'​s chorea and Reye's syndrome, but not in schizophrenics (4-neuroleptic-treated patients) or sudden infant death syndrome. The data presented strongly support the proposal that the measurement of [3H]GABA binding in postmortem human brain offers a reflection of the state of the physiologically relevant GABA receptor.
 +
 +===== ===== 
 +
 +{{gview 100%,​450px>​ http://​directlinks.cc/​files/​muscimol/​218679.pdf}}
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 +Author List: Lloyd K G, Dreksler S
 +
 +Publication Types: Journal Article
 +
 +Substances mentioned in the article: Receptors, Neurotransmitter;​ gamma-Aminobutyric Acid; 
 +
 +Mesh terms: Adolescent; Adult; Aged; Binding Sites/drug effects; Binding, Competitive/​drug effects; Brain/​metabolism;​ Caudate Nucleus/​metabolism;​ Cerebellar Cortex/​metabolism;​ Female; Globus Pallidus/​metabolism;​ Hippocampus/​metabolism;​ Humans; Huntington Disease/​metabolism;​ Male; Middle Aged; Putamen/​metabolism;​ Receptors, Neurotransmitter/​drug effects; Reye Syndrome/​metabolism;​ gamma-Aminobutyric Acid/​metabolism; ​
 +
 +{{tag> Receptors,​_Neurotransmitter gamma-Aminobutyric_Acid }}
 +
 +{{keywords>​ Receptors, Neurotransmitter,​ gamma-Aminobutyric Acid}}
 +
  
218679.txt ยท Last modified: 2018/11/22 21:16 (external edit)