2364289

Corticosteroids regulate the gene expression of FGF-1 and FGF-2 in cultured rat astrocytes.

Article date: 2001/2/24

PubMed ID: 2364289

Journal name: Journal of molecular neuroscience : MN (ISSN: 0895-8696)

ABSTRACT

The present data show that the gene expression of FGF-1 and FGF-2 is regulated by corticosteroids in rat type 1 astrocytes. In particular, the gene expression of FGF-1 is modulated by corticosteroids acting both on type I (minerocorticoid) and type II (glucocorticoid) receptors. In fact, at short times of exposure (2 h) a slight decrease in FGF-1 mRNA levels is induced by deoxycorticosterone, a steroid able to interact with the type I receptors; a similar effect is observed at 6 h following exposure to corticosterone or its 5alpha-reduced metabolite, dihydrocorticosterone. Conversely, at longer times of exposure (24 h) corticosterone is able to strongly increase FGF-1 mRNA levels. Both effects of corticosterone (inhibition and stimulation) were duplicated by dexamethasone, indicating that both effects occur via the type II receptors. Interestingly, the 5alpha-3alpha-reduced metabolite of deoxycorticosterone, tetrahydrodeoxycorticosterone, which does not interact with either corticosteroid receptors, is able to stimulate (at 6 and 24 h of exposure) the gene expression of FGF-1. It is possible that this effect might be induced via the GABA(A) receptor, since muscimol, an agonist of this receptor, exerts a similar effect. The situation is different in the case of FGF-2. The mRNA levels of this growth factor are only stimulated by steroids interacting with type II receptors. Altogether, these observations indicate that corticosteroids modulate the levels of FGF-1 and FGF-2 gene expression in astroglial cells by interaction with classical (type I and II) or nonclassical (GABA(A) receptor) steroid receptors.

Author List: Magnaghi V, Riva M A, Cavarretta I, Martini L, Melcangi R C

Publication Types: Journal Article; Research Support, Non-U.S. Gov't

Substances mentioned in the article: Adrenal Cortex Hormones; GABA Agonists; RNA, Messenger; Receptors, GABA-A; Receptors, Glucocorticoid; Fibroblast Growth Factor 2; Fibroblast Growth Factor 1; Muscimol;

Mesh terms: Adrenal Cortex Hormones/metabolism; Animals; Animals, Newborn; Astrocytes/drug effects; Cells, Cultured/drug effects; Fibroblast Growth Factor 1; Fibroblast Growth Factor 2/genetics; GABA Agonists/pharmacology; Gene Expression Regulation/drug effects; Muscimol/pharmacology; RNA, Messenger/drug effects; Rats; Rats, Sprague-Dawley; Receptors, GABA-A/drug effects; Receptors, Glucocorticoid/drug effects;

Citations: - 10350636

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