Methylmercury modulates GABAA receptor complex differentially in rat cortical and cerebellar membranes in vitro.

Article date: 1995/6/1

PubMed ID: 2436355

Journal name: Neurochemical research (ISSN: 0364-3190)


Effects of methylmercury (MetHg) on the specific [3H]flunitrazepam binding were studied in rat cortical and cerebellar P2-fractions in vitro. MetHg did not affect significantly the specific [3H]flunitrazepam binding in unwashed P2-fraction but increased it marginally (by 16%) at 100 microM in washed P2-fraction, in both brain regions. Muscimol (3 microM), a GABAA agonist, stimulated the [3H]flunitrazepam binding by 30% to 50% depending on the brain region. In washed cerebellar membranes the enhancing response of muscimol was 10 to 14% lower after preincubation of the tissue with MetHg but in cerebral cortex MetHg did not modulate the muscimol response at all. The results indicate that Met-Hg may have region specific effects on GABAA receptors in vitro and the effect may depend on the occupational state of the GABA binding domain of the receptor complex.

Author List: Komulainen H, Keränen A, Saano V

Publication Types: Comparative Study; Journal Article

Substances mentioned in the article: Methylmercury Compounds; Receptors, GABA-A; Muscimol; Flunitrazepam; methylmercuric chloride;

Mesh terms: Animals; Cell Membrane/metabolism; Cerebellum/metabolism; Cerebral Cortex/metabolism; Flunitrazepam/metabolism; Kinetics; Male; Methylmercury Compounds/pharmacology; Muscimol/pharmacology; Neurons/metabolism; Organ Specificity; Rats; Rats, Wistar; Receptors, GABA-A/drug effects;

Citations: - 46771

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