Article date: 1987/1/1
PubMed ID: 2447905
Journal name: Alcohol and alcoholism (Oxford, Oxfordshire). Supplement (ISSN: 1358-6173)
Several lines of research suggest that the depressant effects of intoxicant-anesthetic compounds are mediated through the GABA receptor-chloride ionophore. We have recently demonstrated a procedure that allows direct measurement of GABA-mediated chloride influx in neural tissue. Using this approach in conjunction with receptor-binding techniques, we examined the role of the GABAergic system using mice selected for genetic differences in ethanol hypnosis. A low concentration of ethanol (15 mM) enhanced the muscimol-stimulated chloride influx in cerebellar membranes from Long-sleep (LS-ethanol sensitive) mice, but had no effect on membranes from Short-sleep (SS-ethanol resistant) mice. In addition, muscimol was more potent in stimulating chloride flux and in inhibiting 35S-TBPS binding in LS membranes than in SS membranes. Mice from a heterogeneous stock were selected for differences in ethanol-induced loss of righting reflex. Cerebellar membranes from these mice also showed differences in ethanol augmentation of muscimol-stimulated chloride flux in vitro that were related to their differential sensitivity to ethanol in vivo. This combined genetic-neurochemical approach provides strong evidence for the importance of GABA-operated chloride channels in ethanol intoxication.
Author List: Allan A M, Huidobro-Toro J P, Bleck V, Harris R A
Publication Types: Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.
Substances mentioned in the article: Chlorides; Ion Channels; Receptors, GABA-A; Muscimol; Ethanol;
Mesh terms: Animals; Brain/drug effects; Chlorides/metabolism; Ethanol/pharmacology; Ion Channels/drug effects; Male; Mice; Muscimol/pharmacology; Receptors, GABA-A/drug effects;