Article date: 1989/1/1
PubMed ID: 2471607
Journal name: Comparative biochemistry and physiology. C, Comparative pharmacology and toxicology (ISSN: 0742-8413)
1. The effects of ivermectin (IVM) and milbemycin D (MMD) on cultured embryonic spinal neurons were investigated, using the whole-cell recording technique. 2. IVM and MMD caused Cl- current under voltage-clamp conditions. 3. The reversal potential of the MMD-evoked Cl- current was shifted in a positive direction when half of the external Cl- was substituted by isethionate. 4. The MMD-evoked current was inhibited by picrotoxinin and bicuculline, whereas it was enhanced by diazepam. 5. Different characteristics of desensitization were observed between the MMD- (IVM-) and muscimol-induced effects. 6. These results suggest that IVM and MMD directly activate the Cl- channel through their specific sites modulated by GABAergic drugs.
Author List: Yamazaki J, Matsumoto K, Ono H, Fukuda H
Publication Types: Journal Article; Research Support, Non-U.S. Gov't
Substances mentioned in the article: Anti-Bacterial Agents; Chlorides; Ion Channels; Lactones; Macrolides; Picrotoxin; Muscimol; milbemycin; gamma-Aminobutyric Acid; Ivermectin; picrotoxinin; Diazepam; Bicuculline;
Mesh terms: Animals; Anti-Bacterial Agents/pharmacology; Bicuculline/pharmacology; Cells, Cultured; Chick Embryo; Chlorides/metabolism; Diazepam/pharmacology; Ion Channels/drug effects; Ivermectin/pharmacology; Lactones/pharmacology; Macrolides; Membrane Potentials/drug effects; Muscimol/pharmacology; Neurons/drug effects; Picrotoxin/analogs & derivatives; Spinal Cord/cytology; gamma-Aminobutyric Acid/metabolism;