2538560

Effects of picrotoxin treatment on GABAA receptor supramolecular complexes in rat brain.

Article date: 1989/4/1

PubMed ID: 2538560

Journal name: Journal of neurochemistry (ISSN: 0022-3042)

ABSTRACT

The effects of acute and chronic administration of a subconvulsive dose of picrotoxin on t-[35S]butylbicyclophosphorothionate ([3S]TBPS), [3H]muscimol, and [3H]flunitrazepam binding characteristics in various regions and on the convulsant potency of picrotoxin in Sprague-Dawley rats were examined. Acute administration of a subconvulsive dose of picrotoxin (3 mg/kg, i.p.) significantly increased [35S]TBPS and [3H]muscimol binding in cerebellum (CB) with no change in frontal cortex (FC). In rats treated chronically with picrotoxin (3 mg/kg, i.p., daily for 10 days), the Bmax of [35S]TBPS binding site was significantly decreased in the FC, striatum (ST), and CB with no change in KD values. Neither [3H]muscimol binding in the FC and CB nor [3H]flunitrazepam binding in the FC was affected in these rats. In addition, the potency of pentobarbital to inhibit [35S]TBPS binding in vitro was not altered following acute or chronic treatment of picrotoxin. Chronic administration of picrotoxin did not affect convulsive ED50 or LD50 of picrotoxin; however, it delayed the onset of convulsions and increased the time to death. These results suggest that treatment with picrotoxin at a subconvulsive dose for 10 days causes down-regulation of [35S]TBPS binding sites and that this down-regulation might be related, at least in part, to the decreased extent of convulsant potency of picrotoxin. In addition, the results indicate possible interaction between convulsant binding sites and GABAA receptor sites in the CB following picrotoxin treatment.

This document is available from: http://directlinks.cc/files/muscimol/2538560.pdf

Author List: Ito Y, Lim D K, Nabeshima T, Ho I K

Publication Types: Journal Article; Research Support, U.S. Gov't, P.H.S.

Substances mentioned in the article: Bridged Bicyclo Compounds; Bridged Bicyclo Compounds, Heterocyclic; Receptors, GABA-A; Picrotoxin; Muscimol; Flunitrazepam; tert-butylbicyclophosphorothionate;

Mesh terms: Animals; Brain/metabolism; Bridged Bicyclo Compounds/metabolism; Bridged Bicyclo Compounds, Heterocyclic; Dose-Response Relationship, Drug; Flunitrazepam/metabolism; Male; Muscimol/metabolism; Picrotoxin/pharmacology; Rats; Rats, Inbred Strains; Receptors, GABA-A/drug effects; Seizures/diagnosis; Tissue Distribution;

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