Article date: 1989/2/27
PubMed ID: 2541384
Journal name: Neuroscience letters (ISSN: 0304-3940)
gamma-Aminobutyric acid (GABA)/benzodiazepine receptors purified from the brains of young and adult rats were photolabeled by [3H]flunitrazepam or [3H]muscimol. Gel electrophoresis revealed 3 different proteins with apparent molecular weight (Mr) 51,000, 53,000 and 59,000 which were specifically and irreversibly labeled by [3H]flunitrazepam and recognized by the alpha-subunit specific antibody bd-28. Similarly, 3 different proteins with Mrs 51,000, 53,000 and 56,000 were irreversibly labeled by [3H]muscimol and recognized by the beta-subunit-specific antibody bd-17. Comparison of the photolabeling and immunological staining pattern from young and adult rats seems to indicate that proteins labeled by [3H]flunitrazepam and bd-28 are different from those labeled by [3H]muscimol and bd-17.
Author List: Fuchs K, Sieghart W
Publication Types: Journal Article; Research Support, Non-U.S. Gov't
Substances mentioned in the article: Affinity Labels; Receptors, GABA-A; Muscimol; Flunitrazepam;
Mesh terms: Affinity Labels; Animals; Blotting, Western; Brain/metabolism; Electrophoresis, Polyacrylamide Gel; Flunitrazepam; Immunoenzyme Techniques; Muscimol; Rats; Receptors, GABA-A/analysis;