Article date: 1989/1/1
PubMed ID: 2549321
Journal name: Life sciences (ISSN: 0024-3205)
Chronic alcohol ingestion during pregnancy can lead to the Fetal Alcohol Syndrome (FAS), a disorder marked by learning disabilities. A rat model of FAS was used by introducing pregnant Sprague-Dawley rats to a liquid diet containing 35% ethanol-derived calories (E), while a second group was pair-fed an isocaloric liquid diet without ethanol (P). A third group of pregnant dams received ad libitum lab chow (C). At parturition, pups from the E and P groups were cross-fostered by C mothers and all groups received lab chow. During adulthood, male offspring were sacrificed and hippocampal and prefrontal cortical slices were prelabeled with [3H] inositol. Phosphoinositide (PI) hydrolysis was determined by measuring the accumulation of [3H]inositol phosphates in the presence of LiCl in response to activation of various excitatory amino acid (EAA) receptors. In hippocampal slices, ibotenate- and quisqualate-induced PI hydrolysis was reduced in E compared to P and C animals. Moreover, the inhibitory effect of N-methyl-D-aspartate (NMDA) on carbachol-induced PI hydrolysis, evident in P and C animals, was completely abolished in the hippocampus of E animals. In contrast, in the prefrontal cerebral cortex, this inhibitory effect of NMDA prevailed even in the E animals. The evidence suggest that prenatal ethanol exposure alters the activity of EAA receptors in the hippocampal generation of 2nd messengers.
Author List: Noble E P, Ritchie T
Publication Types: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.
Substances mentioned in the article: Amino Acids; Oxadiazoles; Phosphatidylinositols; Receptors, AMPA; Receptors, Amino Acid; Receptors, Cell Surface; Receptors, Neurotransmitter; Ibotenic Acid; Ethanol; Quisqualic Acid;
Mesh terms: Amino Acids/metabolism; Animals; Disease Models, Animal; Ethanol/toxicity; Female; Fetal Alcohol Spectrum Disorders/metabolism; Hippocampus/metabolism; Ibotenic Acid/metabolism; Male; Oxadiazoles/metabolism; Phosphatidylinositols/metabolism; Pregnancy; Prenatal Exposure Delayed Effects; Quisqualic Acid; Rats; Rats, Inbred Strains; Receptors, AMPA; Receptors, Amino Acid; Receptors, Cell Surface/metabolism; Receptors, Neurotransmitter/metabolism;