Article date: 1989/9/1
PubMed ID: 2561970
Journal name: Neuron (ISSN: 0896-6273)
Two cDNAs encoding novel GABAA receptor subunits were isolated from a rat brain library. These subunits, gamma 2 and delta, share approximately 35% sequence identity with alpha and beta subunits and form functional GABA-gated chloride channels when expressed alone in vitro. The gamma 2 subunit is the rat homolog of the human gamma 2 subunit recently shown to be important for benzodiazepine pharmacology. Cellular localization of the mRNAs encoding the gamma 2 and delta subunits in rat brain revealed that largely distinct neuronal subpopulations express the two subunits. The delta subunit distribution resembles that of the high affinity GABAA receptor labeled with [3H]muscimol; the gamma 2 subunit distribution resembles that of GABAA/benzodiazepine receptors labeled with [3H]flunitrazepam. These findings have implications for the composition of two different GABAA receptor subtypes and for information processing in networks using GABA for signaling.
Author List: Shivers B D, Killisch I, Sprengel R, Sontheimer H, Köhler M, Schofield P R, Seeburg P H
Publication Types: Journal Article
Substances mentioned in the article: GABRG2 protein, human; Gabrg2 protein, rat; RNA, Messenger; Receptors, GABA-A; DNA;
Mesh terms: Animals; Base Sequence; Cloning, Molecular; DNA/genetics; Humans; Molecular Sequence Data; Neurons/metabolism; RNA, Messenger/metabolism; Rats; Receptors, GABA-A/analysis;