Article date: 1989/8/7
PubMed ID: 2569917
Journal name: Brain research (ISSN: 0006-8993)
The actions of the excitatory amino acid ibotenate were investigated in postnatal rat hippocampal neurons. In neurons voltage clamped at negative membrane potentials using low-chloride internal solutions, ibotenate responses consist of an inward cationic current and two outward currents. The inward current is inhibited by 2-amino-5-phosphonovalerate (APV), a selective N-methyl-D-aspartate (NMDA) antagonist. The two outward currents consist of a picrotoxin and bicuculline-sensitive chloride current and a slowly developing calcium activated potassium current. The bicuculline sensitive current appears to be the product of contamination of ibotenate samples with the gamma-aminobutyric acid (GABA) agonist muscimol and not the result of a direct action of ibotenate.
Author List: Zorumski C F, Todd R D, Clifford D B
Publication Types: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.
Substances mentioned in the article: Oxazoles; Receptors, N-Methyl-D-Aspartate; Receptors, Neurotransmitter; Ibotenic Acid; 2-Amino-5-phosphonovalerate; Valine;
Mesh terms: 2-Amino-5-phosphonovalerate; Action Potentials/drug effects; Animals; Cells, Cultured; Hippocampus/drug effects; Ibotenic Acid/pharmacology; Oxazoles/pharmacology; Rats; Receptors, N-Methyl-D-Aspartate; Receptors, Neurotransmitter/drug effects; Valine/analogs & derivatives;