Cholinergic and noradrenergic denervations decrease labelled purine release from electrically stimulated rat cortical slices.

Article date: 1989/1/1

PubMed ID: 2601836

Journal name: Neuroscience (ISSN: 0306-4522)


The origin of cortical purine release was investigated by measuring [3H]purine release from electrically stimulated cortical slices of rats after neurotoxic lesions of cholinergic, noradrenergic and serotoninergic pathways innervating the cortex. Purines were labelled by incubating the cortical slices with [3H]adenine. The 3H efflux at rest and during stimulation, analysed by high performance liquid chromatography, consisted of adenosine, inosine, hypoxanthine and a small amount of nucleotides. Twenty days after unilateral or bilateral lesion of the nucleus basalis a marked decrease in choline acetyltransferase activity was associated with a decrease in [3H]purine release. A linear relationship was found between the decrease in choline acetyltransferase activity and [3H]purine release. A partial recovery in both choline acetyltransferase activity and [3H]purine release was observed eight months after the lesion. Twenty days after intra-cerebroventricular injection of 6-hydroxydopamine a 59% decrease in cortical noradrenaline content was associated with a 44% decrease in [3H]purine release. Conversely, no change in [3H]purine release was found in rats in which a 89% decrease in cortical serotonin content was induced by intra-cerebroventricular injection of 5,7-dihydroxytryptamine. The decrease in [3H]purine release after the lesion of the cholinergic and noradrenergic pathways may depend on metabolic changes, a loss of a stimulating influence of acetylcholine and noradrenaline or may indicate a release of [3H]purine from cholinergic and noradrenergic fibres.

Author List: Pedata F, Di Patre P L, Giovannini M G, Pazzagli M, Pepeu G

Publication Types: Journal Article; Research Support, Non-U.S. Gov't

Substances mentioned in the article: Hypoxanthines; Purines; Ibotenic Acid; Hypoxanthine; Inosine; Choline O-Acetyltransferase; Adenosine;

Mesh terms: Adenosine/metabolism; Adrenergic Fibers/enzymology; Animals; Basal Ganglia/physiology; Cerebral Cortex/metabolism; Choline O-Acetyltransferase/metabolism; Cholinergic Fibers/physiology; Electric Stimulation; Hypoxanthine; Hypoxanthines/metabolism; Ibotenic Acid; Inosine/metabolism; Male; Nerve Degeneration; Purines/metabolism; Rats; Rats, Inbred Strains;

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